November 2017 Vol. 5 No.11

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El-Badry MA
Sheir DH

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Original Research Article

Microbial Fungal Community Fingerprint of Medicinal Plant Rhizosphere Collected Form Sinai, Egypt

M. A. El-Badry^1* and Donia H. Sheir²

¹Botany and Microbiology Department, Faculty of Science, Al-Azher University, Egypt
²Department of Natural and Microbial Products, National Research Center, Egypt

*Corresponding Author’s E-mail: mohamed.helwanuni@yahoo.com
 
Accepted November 19, 2017

Abstract

The rhizosphere is a narrow region of soil that is directly affected by roots and associated soil microorganisms. Research on rhizosphere microbes of various medicinal plants is essential for microbial ecology, applied microbiology and industrial biotechnology with regard to the sustainable utilization of egyption medicinal resources. However, the inability of culturing most rhizosphere microorganisms (around 99%) in the laboratory obviates the research progress. In recent years, there are enormous advances in applying non-culturing techniques based on molecular biology and omics to the study of rhizosphere microbial diversity and plant-microbe interactions terminal restriction fragment length polymorphism (T-RFLP). Dry Rhizosphere of medicinal plants collected form Sinai, Egypt and water treated represents a common physiological stress for the microbial communities residing in surface of these medicinal plants. A dry and wetting by water induce lysis in a significant proportion of the microbial biomass and, for a number of reasons, Effect directly or indirectly on microbial community composition. In this study Dry sand and water treated as common stress in the laboratory by exposing three different rhizosphere immersed in water to 10 day and 20 day period. The three rhizospheric medicinal plants were collected from Saint Katherine Mountain, Sinai, Egypt. Fungal community of dry and immersed with distilled water was evaluated every 10 day of incubation. Total DNA was extracted from sand samples and characterized its fungal communities using the T-RFLP method. This work revealed that water changed fungal community in all samples compared with dry sand due to water may induced shifts in bacterial community.

Keywords: Microbial fingerprint, bacterial community, TRFLP, medicinal plants, soil DNA extraction, 16S ribosomal RNA