October 2017 Vol. 5 No.10

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Original Research Article

How Scaffolds, which are Polymeric Drug Delivery Systems Allowing Controlled Release, can be Tested in Human Primary Nucleus Pulposus and Annulus Fibrosus Cell Cultures?

Yener Akyuva¹, Numan Karaarslan^2*, Ibrahim Yılmaz³, Hanefi Ozbek⁴, Duygu Yasar Sırın⁵, Mehmet Sabri Gurbuz⁶, Yasin Emre Kaya⁷, Necati Kaplan⁸ and Ozkan Ates⁹

¹M.D. Gaziosmanpasa Taksim Training and Research Hospital, Department of Neurosurgery, 34433, Istanbul, Turkey
²Assist. Prof. M.D.; Namik Kemal University School of Medicine, Department of Neurosurgery, 59100, Tekirdag, Turkey
³Medical Pharmacologist, Pharmacist; Istanbul Medipol University, School of Medicine, Department of Medical Pharmacology, 34810, Istanbul, Turkey
⁴Prof. M.D., Ph.D.; Istanbul Medipol University, School of Medicine, Department of Medical Pharmacology, 34810, Istanbul, Turkey
⁵Assist. Prof. Ph.D.; Namik Kemal University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, 59100, Tekirdag, Turkey
⁶Assist. Prof. M.D., Istanbul Medeniyet University School of Medicine, Department of Neurosurgery, 34000, Istanbul, Turkey
⁷Assist. Prof. M.D., Abant Izzet Baysal University School of Medicine, Department of Orthoaedic and Traumatology, 14000, Bolu, Turkey
⁸Assist. Prof. M.D., Istanbul Rumeli University, Corlu Reyap Hospital, Department of Neurosurgery, 59860, Tekirdag, Turkey
⁹Prof. M.D., EsenyurtUniversity, EsencanHospital, Department of Neurosurgery, 34517, Istanbul, Turkey

*Corresponding Author’s Émail: numikara@yahoo.com
Pbx: +9028 2250 5500

Accepted September 28, 2017

Abstract

Promising pharmacological treatment of disk degeneration depends on development of appropriate materials and obtaining viable and proliferative nucleus pulposus/annulus fibrosus tissues with preservation of extracellular matrix compounds. This study aims to explain how drug and/or similar material impregnated polymeric scaffolds can be tested using primary cell cultures derived from human nucleus pulposus and annulus fibrosus tissues at pharmacomolecular level. For this purpose; we first explained how to prepare human primary cell culture setups. We remarked the physicochemical parameters for testing drugs and scaffolds containing drug-like substances. How to conduct the molecular analyzes of samples of cell cultures was explained. In this study; we explained very basically how to make in vitro analyzes for the development of the ideal drugs and/or polymeric biomaterials that can be used in clinical practice for the purpose of preventing disc degeneration. Moreover, medical pharmacologists, orthopedic and brain surgeons taking part in such studies can set up and analyze their own studies.

Keywords: Annulus fibrosus/ nucleus pulposus culture, controlled release, polymeric scaffolds