August 2015 Vol. 3 No. 8

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Original Research Article

The role of lesser grain borer in transmission of pathogenic bacteria to poultry and animal feed

Erfan^1* A. M., Marouf² S. H. and Rezk³ A.

¹Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Dokki- Giza
²Microbiology Department, Faculty of Vet Medicine, Cairo University
³Veterinary Hygiene and Management Department, Faculty of Veterinary Medicine, Cairo University

*Corresponding Author’s E-mail: erfan8279ahmed@yahoo.com
Tel.: 00233380121
Cell.: 00201006498433
Fax: 00233370957

Accepted May 11, 2015

Abstract

Insects have been considered as the most diverse species of animals living on earth. The majority of insects were directly related to humans, animal and to the environment. The goal of the present study was to detect the role of lesser grain borer as stored product insect in harboring and potentiating virulent bacteria. The study included both whole and homogenized form of insect for microbial detection where S. equi, Streptococcus pyogenes, S. aureus, S. epidermidis, Salmonella, E. coli and Enterococcus were identified. The positive PCR results obtained for eqbe, smeZ, clfA, gseA, invA, phoA and 16SrRNA genes were capable to confirm the isolation of these microbes, respectively. The virulence profile of the isolated bacteria was strengthened by the positive results encountered for the different virulence genes tested by PCR. The isolated bacteria were resistant to almost all tested antibiotics.

Keywords: E. coli, Enterococcus, Lesser grain borer, Salmonella, Staphylococcus