August 2015 Vol. 3 No. 8

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Original Research Article

Molecular characterization and antibiotic resistance of bacterial pathogens from respiratory tract infection

*Nazish Mazhar Ali, Mudassar Hussain, Syed Abdullah Mazhar, Bushra Mazhar, Bushra Kalim, Safia Rehman and
Muhammad Shahzad
 

Microbiology and Molecular Biology Laboratory, Department of Zoology, GCU Lahore

*Corresponding Author’s Email: nazipak@hotmail.com

Accepted July 25, 2015

Abstract

In the present study the bacteria were isolated from sputum samples of patients suffering from respiratory tract infection. These virulent bacteria were checked for antibiotic resistance. All antibiotic resistant bacteria were biochemically characterized at genus level (Streptococcus sp. and Pseudomonas sp.). Three bacterial strains were confirmed by ribotyping at species level named Bacillus cereus, Klebsiella pneumoniae and Pseudomonas putida. The Results indicated that all the strains were highly resistant against Cloxacillin, Amoxyllin/Clavulanic acid, Oxacillin, Cefepime and Am-picillin. Streptococcus sp. and Pseudomonas sp. were also having resistance potential against Penicillin. Penicillin is not effective against Pseudo-monas sp. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were also checked against azithromycine, doxycycline, cephalexin, cefepime, tetracycline, gentamicin and amikacin for all bacterial species. The highest MIC of Bacillus cereus was observed as 78.1µg/ml against Gentamicin. Like Bacillus cereus, Klebsiella pneumoniae was also having highest MIC 78.1µg/ml against Gentamicin. 48.8µg/ml tetracycline was examined the higher MIC for Pseudomonas putida. The MBC against antibiotics were also observed for bacterial strains and most of these MIC values were unchanged while others were high. From this study, it was concluded that the antibiotic resistant bacteria were progressively increased due to continuous exposure to same antibiotics. This increase of MIC and MBC of various antibiotics against bacterial species was seemed due to their higher drug resistance potential.

Key words: Antibiotics, Bacillus cereus, Klebsiella pneumoniae, Pseudomonas putida, Respiratory tract infection, Ribotyping