November 2017 Vol. 5 No.11

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Short Communication

Establishment of campylobacter infection with immunochromatographic test

Valeri Velev^1*, Maria Pavlova², Atanas Mangarov¹, Ivan Ivanov² and Todor Kantardjiev²

¹Department of Infectious Diseases and Parasitology, Medical University of Sofia
²Nacional Centre of Infectious and Parasitic Diseases, Sofia

*Corresponding Author’s E-Mail: velev_md@abv.bg

Accepted September 18, 2017

Abstract

Campylobacter spp. are the most commonly reported bacterial causes of food toxic infections in the world. Traditionally, microorganisms of the genus Campylobacter are difficult to detect because they require a special incubation medium under micro-aerophilic conditions. To introduce mass immunochromatographic tests in diarrheal patients, verifying their diagnostic value with that of a molecular-biological method optimized by us. Fecal samples of 520 children with diarrhea syndrome we retested by immunochromatography (ICT) and Eva Green Real-time PCR for simultaneous differentiation of C. jejuni/coli directly from feces. We found a statistically significant difference between the two diagnostic tests at Sig. <0,05 and t >3. Using calculator for value of the Cohen's d (reflecting the magnitude of the difference between two variables) we calculated Cohen's d = 0.346, which in fact suggests that the difference between the diagnostic capabilities of the ICT and the Eva Green Real-time PCR methodis very small. The diagnostic capabilities of modern ICT are significant and the work with them can significantly ease the activity of the clinician in the necessity to make quick decisions about the therapeutic behavior.

Key words: Campylobacter, ICT, PCR