May 2017 Vol. 5 No.5

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Original Research Article

Identification, molecular characterization and phylogenetic analysis of cytochrome c oxidase gene from bacterial-infected Culex pipiens (Diptera: Culicidae)

Mohamed Z. Y. Aly¹, Khalid S. M. Osman¹, Fatma H. Galal*^2,3 and Salwa I. Sebak¹

¹Department of Zoology, Faculty of Science, South Valley University
²Department of Biology, College of Science, Aljouf University
³Department of Entomology, Faculty of Science, Cairo University

*Corresponding Author’s E-mail: fatmahgalal@yahoo.com / fhgalal@ju.edu.sa

Accepted May 10, 2017

Abstract

The present work aims to identify, molecular characterization and phylogenetic analysis of the induced antibacterial gene, cytochrome c oxidase, from the whole body of bacterial-fed fourth instar Cx. Pipiens larvae using differential display technique. For achievement this research, the fourth instar Cx. Pipiens larvae were fed on gram (+) bacteria, S. aureus, gram (-) bacteria, K. neumonia and mix. Whole body infected larvae were collected at 6, 12, 18, 24, 30, 36, 42, 48, 54, 60, 66 and 72 h postfeeding. The differential display technique was employed to screen the genetic variation (at RNA level) between bacterial-fed and control fourth instar Cx. Pipiens larvae. Nine reproducible bands were eluted and sequenced to characterize the full length cDNA of the induced genes. The results indicated the presence of differentially displayed bands in the bacterial- fed larvae and not observed in controls. The resulting sequences were blasted to cytochrome c oxidase.

Keywords: antibacterial gene, Cx. Pipiens, cytochrome c oxidase, DD-PCR, immune response