March 2017 Vol. 5 No.3

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Original Research Article

Repeated intratracheal instillation of TiO₂ nanoparticles effect on MMP-12 expression in rat’s lung

Dhamia K. Suker¹, Fatimah A. Jasim^1* and Saad Abdullah³

¹University of Basra, College of Science, cell and biotechnology research unit
²Ministry of Education
³University of Basra, College of medicine

*Corresponding Author’s E-mail: alifaith28@yahoo.com

Accepted March 06, 2017

Abstract

Titanium dioxide (TiO₂) nanoparticles have biological effects that may be caused by fibrosis or emphysema through effects on matrix metalloproteinase expression. The aim of this study investigates the effect of TiO₂ nanoparticles on MMP-12 expression. Sixty-three rats were intratracheally instilled with (0.5, 1.5, 5, 15, 50, 150) mg/kg body weight of TiO₂ nanoparticles for 4 weeks. Animals were sacrificed at 4 days, a month and 3 months post-instillation, immunohistochemistry of MMP-12 antibody and lung mRNA of MMP-12 gene expression. At 4 days post-instillation, TiO₂ nanoparticles decreased macrophage MMP-12 expression with emphysema at high doses and fibrosis at low doses caused fibrosis while after a month and 3 months post-instillation macrophage MMP-12 increased and induce pulmonary response with emphysema at low doses. TiO₂ nanoparticles aggravated elastase-induced emphysema and caused histological inflammation and downregulate MMP-12 mRNA expression and protein expression in macrophage and matrix extracellular.

Keywords: MMP-12 expression, TiO₂ nanoparticles effects, MMP-12 immunostaining, emphysema