May 2021 Vol.9 No.2

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Original Research Article

Phenotypic and genotypic detection of extended-spectrum b-lactamase (ESBLs) production of E.coli isolated from urine samples in Al-Anbar province/Iraq

Essa Saeed¹, Özgür Kuzukiran², Jaleel Samanje^3*

¹M.Sc. Çankiri Karatekin University, Biology University, Biology Department, Turkey
²Çankiri Karatekin University-Eldivan Vocational School of Health Services –Veterinary Departmant,Turkey.
³Collage of Health and Medical Technology, Middle Technical University, Baghdad, Iraq

*Corresponding author Email: jaleel.najah@mtu.edu.iq

Received: 18 April 2021    I    Accepted: 23 May 2021    I    Published: 31 May 2021    I    Article ID: MRJMBS21015
Copyright © 2021 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0.

Abstract

The present study was designed to determine phenotyping antibiotic resistance patterns among patients infected with uropathogenic E. coli admitted to hospitals at Al-Anbar province/Iraq and study the frequency of (blaCTX-M, blaTEM and blaOXA) genes among the extended spectrum β-lactamase ESBL producing isolates. The results showed that (52) out of (65) uropathogenic E.coli isolates were recognized to be ESBL producers through the use of combination disc test (CDT) and vitek system. The frequency of β-lactamase (bla) genes (blaCTX-M, blaTEM and blaOXA) of the E.coli were molecularly identified using the Polymerase Chain Reaction technique (PCR). In addition, 29(55.76%) of the 65 phenotypically ESBL-producing uropathogenic were selected and subjected to gene detection, and 29/29 (100%) tested positive for blaTEM and blaCTX-M respectively, whereas E.coli recorded 0/29 (0%) negative result of blaOXA gene. By VITEK system, the isolated E.coli observed variable resistance rate toward ESB antibiotic groups such as (91%) to Ticarcillin, (91%) to Piperacillin, (91%) to Ceftazidime, (91%) to Cefepime, and (12.13%) to Imipenem. While the isolated E.coli showed susceptibility to Piperacillin/ tazobactam (69.69%). The current study revealed the presence of CTX-M and TEM genes among uropathogenic E. coli strains in Al-Anbar province/Iraq. While blaOXA gene first time in Iraq with (0%) negative result . Although molecular methods are more reliable in ESBL production identification, it is necessary to conduct routine clinical examination to identify ESBL-producing uropathogenes by introducing phenotypic methods which must be encouraged in clinical setting since they are cheaper methods, and they will help in testing the drug resistance.

Keywords: Antibiotic, CDT, CTX-M,ESBLs, E.coli, TEM, VITEK