January 2016 Vol.4 No.1

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Original Research Article

Prevalence of bacterial isolates possibly transmitted by Musca demostica from different areas of Damanhur City, El behara, Egypt and its impact on Musca demostica cuticle

El Ahwany A.M.D.¹, Radwan E.H.², Beltagy B.³ and Abo El Nga O.A.⁴

¹Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt
²Zoology Department, Faculty of Science, Damanhur University, Egypt
^3,4Zoology Department, Faculty of Science, Alexandria University, Egypt

*Corresponding Author’s E-mail: dr_eman_hashem@yahoo.com

Accepted September 07, 2015

Abstract

The house-fly, Musca domestica L., not only is a nuisance pest, but also it acts as an important mechanical vector for lots of pathogenic microorganism agents, including: bacteria, protozoa, worms, fungi and viruses amongst humans and animals. The aim of this study was to isolate and identification bacteria that are pick up by house-fly over the human and animal premises and study their effect on house fly cuticle. In this study house flies were collected from Damanhur El-homiat hospital, Damanhur public garden in addition to those that reared in laboratory as (control group), to isolate their bacteria. The bacteria were identified by DNA extraction and PCR amplification of their 16S rRNA gene. The bacterial isolates included 7 new bacterial records for house flies: Bacillus subtilis, Bacillus cereus, Bacillus flexus, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus thuringiensis and Klebsiella pneumonia. Most of the organisms recovered from the house fly are serious pathogens, known to produce diseases such as food poisoning, diarrhea, pneumonia, urinary tract infection and inflammation of upper respiratory tract. The possible exception is Bacillus thurinigensis; a known pathogen for insects is that only occasionally produces allergic reactions in humans. Also the presence of bacterial flora on the cuticle of house flies causes morphological changes on their cuticle. In winter and spring, house flies that collected from El-homiat hospital shows pigmentation on epicuticle layer, whereas in summer the house flies collected from Homiat hospital shows disturbance on the morphology of cuticle, also in autumn there is increase in thickness of epicuticle of flies that collected from garden.

Key words: Bacteria, Musca domestica, 16SrRNA gene sequence, cuticle, seasons