January 2016 Vol.4 No.1

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Original Research Article

Prokaryotic diversity from the culture-independent taxonomic analysis of a sugarcane bagasse metagenome

Ramón Alberto Batista-García^1,2*, Rocío Casasanero², Alberto Alvárez-Castillo³, Stephen A. Jackson⁴,
Alan D. W. Dobson^4,5 and Jorge Luis Folch-Mallol²

¹Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos. Cuernavaca, Morelos, Mexico.
²Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos. Cuernavaca, Morelos, Mexico.
³Instituto Tecnológico de Zacatepec. Zacatepec, Morelos, Mexico.
⁴Marine Biotechnology Centre, Environmental Research Institute, University College Cork. Cork, Ireland.
⁵School of Microbiology, University College Cork. Cork, Ireland.

*Corresponding Author’s E-mail: rbatista25@yahoo.com, rabg@uaem.mx
+(52) 777-3297057 Ext. 4550
+(52) 7773297057 Ext. 3457

Accepted January 04, 2016

Abstract

A 16S rDNA metagenome library was constructed from sugar bagasse samples and a phylogenetic analysis was conducted. This allowed us to explore the biodiversity present in the bagasse samples and identify novel bacterial groups inhabiting this lignocellulosic rich substrate. The most abundant phyla were Proteobacteria and Acidobacteria, while Candidate division TM7 was the minor phyla. Overall, the prokaryotic microbial diversity revealed the presence of 13 families and 17 genera, with Burkholderiaceae and Burkholderia being the major family and genus observed, respectively. Since we were also able to detect some known bacterial genera, we could identify some specific metabolic pathways that may be important in lignocellulose degradation. Microbial communities inhabiting natural lignocellulosic substrates have to date received little attention, subsequently there is little information about the main bacterial genera present in these substrates resulting in a lack of a comprehensive knowledge about the microbial process involved in lignocellulosic degradation. This study provides some insight into the unique microbial community structure in sugarcane bagasse from Meso Americ (the most important sugar producing region in the world) and is the first such study to characterize these bagasse inhabiting populations.

Keywords: Metagenomic approaches, 16S rDNA biodiversity, cellulolytic environments, bacterial communities.