December 2015 Vol.3 No.3

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Original Research Article

Genetic Variability of Bovine Viral Diarrhea Virus in Persistently Infected Cattle in Central Java, Indonesia

H. Wuryastuti^1*, P. P. Putro¹, R. Wasito¹ and R. K. Maes²

¹Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, Indonesia
²College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, 48823, USA

*Corresponding Author’s E-mail: hastari_ugm@yahoo.com

Accepted December 21, 2015

Abstract

Fifteen bovine viral diarrhea virus (BVDV) positive samples collected from persistently infected (PI) dairy cattle in Central Java, Indonesia, were used to detect the genetic characterization of BVDV based on the 5’-Untranslated Region (5’UTR) of the viral genome. Through phylogenetic and nucleotide sequence analysis of the 5’UTR regions of the samples investigated, it was determined that all the 15 IP-BVDV field positive samples had the BVDV-1 genotype. No evidence of BVDV-2 genotype was found in our recent collected blood samples. The presence of the genetic variability among the BVDV-1 circulating in the dairy cattle population in Central Java, Indonesia was not proven. As a conclusion, further research involving higher number of BVDV-1 positive samples from different region of Indonesia is urgently needed.

Keywords: Bovine viral diarrhea virus, 5’-Untranslated Region, genetic variability, phylogenetic analysis, genotype