March 2015 Vol.3 No.3

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Original Research Article

Effect of Narcissus tazetta extracts on cell cycle and biochemical analysis of Vicia faba

Fawzia I. Mohamed^1*, Zeinab M. El-Ashry¹, Mohamed N. Abd El Gany² and Hamdy H. Abd El Rahman¹

¹Department of Genetic and Cytology, National Research Centre, Dokki, Cairo, Egypt.
²Department of Botany, Faculty of Science, Mansorura University, Egypt.

^*Corresponding Author’s Email: adam_fwz@yahoo.com

Accepted March 26, 2015

Abstract

The aim of the current investigation was to study genotoxicity effect of Naricissus tazetta extracts on DNA image during cell cycle parameters and biochemical analysis in Vicia faba L. plants after treatments the root meristematic cells with different times 3, 6, 12 and 24hrs. The results indicated that, number of cells less than 2C-DNA content significantly decreased after all treatments as compared with control. DNA content or C-value were changed in treated cells. The DNA content 2C revealed a highly significant increase after all treatments. The percentage of maximum value reached to phases obviously altered and was pronounced was pronounced with the decrease in G₀/ G₁ phases and increase S phase after all treatments , in addition change in chromatin structure where, euchromatin decreased with treatment of the extract, this means that Narcissus tazetta extracts block the cell cycle and gene expression. Biochemical analysis revealed the acceptance of genotoxicity tests where, results of the present study indicate the obvious variation in the protein banding patterns of Vicia faba root-tip cells. The recorded changes were expressed as variations in the number of separated bands, disappearance or appearance of certain bands, difference in the intensity of one or more protein polypeptides.

Keywords: Cell cycle, Genotoxicity, Narcissus tazetta, Protein banding, Vicia faba