October 2013 Vol. 1 No. 7

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Full Length Research Paper

Isolation and identification of bioactive compounds from twigs of Artocarpusaltilis

Muhammad Nor Omar¹*, Ahmad Muzammil², Naik T. Khan³, Muhammad Zahid⁴ and Tara Kamal¹

¹Research management center, Office of the Campus Director, International Islamic University Malaysia (IIUM), Jalaln Sultan Ahmad Shah, Bandar InderaMahkota,
25200 Kuantan, Pahang, Malaysia

²Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia (IIUM), Jalaln Sultan Ahmad Shah, Bandar InderaMahkota,25200 Kuantan, Pahang, Malaysia

³Research Officer (Tenure Track), H.E.J Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan

⁴Science Officer Centre for Research and Instrumentation National University of Malaysia

*Corresponding Author's E-mail: noromar@yahoo.com, tarajalal@ymail.com

Accepted November 02, 2013

Abstract

The present study focuses on the bioassay guided isolation of antimicrobial compounds from Artocarpusaltilis. The hexane, dichloromethane and methanol extracts were obtained from the dried (twigs of A. altilis) by using Soxhlet extraction. The plant crude extracts were evaluated for in vitro antimicrobial activities revealed that twigs part of A. altilis had potent antimicrobial activities, further separation had been done through column chromatography (CC) of dichloromethane and hexane extracts of A.altilis, which were afforded stigmasterol and cycloartenyl acetate respectively, the two compounds exhibited in vitro antimicrobial activity against S. aureus value of (11±0.5mm) and (11.3±0.5mm). Whereas minimum inhibition concentration (MIC) and minimum bactericidal/minimum fungicidal concentration (MBC/MFC) for isolated compounds were ranged from 62.5-500 µg/ml. Their structures were identified by ¹H-NMR, ¹³C-NMR, IR and the spectra obtained were compared with previous data. This study forms a strong basis for declaring A. altilisas antimicrobial potential source to treat pathogenic microorganisms.

Keywords: Artocarpusaltilis, antimicrobial, cycloartenylacetat, stigmasterol, MIC, IR, NMR