 |
 |
 |
 |
 |
/ / MRJASSS Home / / About MRJASSS / / Submit Manuscripts / / Call For Articles / / Editorial Board / / Archive / / Author's Guide /
|
Other viewing option
Naeem
M
|
Original Research Article DNA barcoding through mitochondrial CO1 gene: Fish Identification, divergence and phylogenetic studies |
||
|
Dr. Muhammad Naeem and Shoaib Hassan |
|||
|
Professor of
Zoology, Institute of Pure and Applied Biology, Bahauddin
Zakariya University, Multan, 60800, Pakistan |
|||
|
Abstract |
|||
|
DNA barcoding is used to identify particular specie by
Cytochrome c oxidase 1 (CO1) mitochondrial DNA sequence. It is
also deal with genetic variability between species and its
polymorphism. Principally phylogenetic methods based on maximum
likelihood and neighbourhood joining tree for variability in
sequence and taxonomical classification of species. DNA
barcoding is the one means of establishing an effective and
precise system for species identification. A standard short
target of genetic marker often used to craft sequence contour of
identified against unidentified species. Specified marker/tag of
mitochondrial cytochrome C oxidase subunit 1 is used for the
species identification purpose. Usefulness of following method
lay off the divergence degree among species. Compare to
previously used identification method through external or
visible morphology, barcoding is more reliable. Computation of
genetic distances and construction of Neighbor Joining tree
based on the Kimura 2 Parameter method were also done
systematically. Analysis of evolutionary tree stood clearly by
making phylogenetic tree with relationships among their species,
alike species bunching under the same tree node while differing
species clustered under distinct nodes with no exemptions. Study
findings concluded that sequence of Cytochrome C oxidase 1 used
as a milestone to provide identification of species at molecular
level, its similarity and divergence among the related species. |
Merit Research Journals© 2020 || Advertisement | Privacy policy.