March 2018 Vol. 6 No.3

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Karim A
Razzaq A

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Original Research Article

CO1 gene based identification and phylogenetic analysis of two fresh water fish species Labeogonius and Cirrhinusmrigala (family: Cyprinidae) from River Ravi, Punjab, Pakistan

¹Asma Karim, ^2,3Rashid Saif, ¹Zohaib Gill, ¹Faisal Salamat Ali, ¹Rida Fatima and ¹Asem Razzaq

¹Department of Zoology, Govt. College of Science, Wahdat Road, Lahore, Pakistan
²Institute of Biotechnology, Gulab Devi Educational Complex, Ferozepur Road, Lahore, Pakistan
³Decode Genomics, 264- Q, Johar Town, Lahore, Pakistan

*Corresponding Author’s Email: drasmakarim@gmail.com
 
Accepted March 05, 2018

Abstract

Taxonomy has always been a challenging job for the scientists. Traditional methods for identification of fishes are based on morphological features which sometimes create great confusion due to great morphological similarities in closed species. DNA barcoding is a modern species identification method in which a short standardized mitochondrial DNA region is used. In this method sequence diversity in a 658 base pair fragment near the 5′ end of the mitochondrial Cytochrome C Oxidase 1 (CO1) gene is employed as a tool for species identification. This method is capable enough for identification of processed, preserved and damaged fishes as compared to traditional morphometric methods and is also appropriate for rapid identification of juveniles as well as adult stages of fishes. Present study is conducted to identify two fresh water fishes, Labeogonius and Cirrhinusmrigala on basis of CO1 gene. Partial sequences of CO1 gene of these fishes were amplified by conventional PCR method and BLAST analysis confirmed that genetically identified fishes are the same fishes which were identified morphologically at the beginning of the study with the help of identification key. The phylogenetic tree analysis confirmed the evolutionary relationships between L.gonius and C.mrigala from Pakistan and fishes from other parts of the world. From these findings, it is concluded that the partial CO1 gene sequence, may serve as a potential marker for identification of most of fish species at molecular-level and their phylogenetic analysis.

Keywords: DNA barcoding, CO1 gene, Cirrhinusmrigala, Labeogonius, MEGA 7, dnaSP5