April 2018 Vol. 6 No.4

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El-Debaiky E

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Original Research Article

Effect of the new antagonist; Aspergillus piperis on germination and growth of tomato plant and Early Blight incidence caused by Alternaria solani

Samah Abd El-Kader El-Debaiky

Botany Department, Faculty of Science, Tanta University, Egypt

E-mail: Samaheldebaiky@science.tanta.edu.eg
Tel.: 002-01005256803
 
Accepted April 16, 2018

Abstract

The present work is considered the first record of studying the ability of the new antagonist, Aspergillus piperis in decreasing the disease incidence of early blight of tomato plant caused by Alternaria solani. The pathogen A. solani was isolated from naturally diseased tomato fruits and identified genetically by sequencing of rRNA gene using ITS1 and ITS4 primers. For the field experiment, the toxicity of spore suspension of A. piperis on germination of tomato seeds was performed using soaking and irrigation methods where the germination percentage and vigor index were calculated. The results indicated that the irrigation method recorded better results than soaking where the germination percentage was 81.81 % and vigor index was 794.37 related to that of control which recorded 90.9% and 715.83 respectively. The values of vigor index indicated that, the spore suspension of A. piperis had induced the plant growth. In the meantime, the spore suspension of A. piperis was applied on tomato leaflets by several methods to reduce the incidence of early blight disease. The application of A. piperis spore suspension directly on the leaflets exhibited the best result where the percentage of infection was 10.25 % to the control (25 %) after 4 days.

Keywords: Biocontrol agent, Aspergillus piperis, Alternaria solani, Early blight, Tomato, rRNA, ITS