January 2016 Vol. 4 No.1

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Kalwar Q
Nizamani R

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Original Research Article

Evaluation of estrus behavior and fertility rate following estrus synchronization in Kundhi buffaloes

Qudratullah Kalwar^1*, Akeel Ahmed Memon¹, Hamzo Khan Kunbhar¹, Muhammad Bachal Bhutto¹, Amjad Hussain Mirani²,
S. M. H. Andrabi², Asmatullah Kaka¹ and Ali Raza Nizamani¹

¹Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan
²Animal Reproduction Program, Animal Sciences, National Agriculture Research Centre, Park Road, Islamabad, Pakistan

*Corresponding Author's E-mail: akeelahmedmemon@gmail.com

Accepted November 23, 2015

Abstract

The present study was designed to compare the efficacy of estrus synchronization protocols with or without Biostimulation in Kundhi buffaloes at and surroundings of Kundhi buffalo farm, Rorhi. Selected forty Buffaloes (n=40) of 1^st to 5^th parity were divided into group A (Bull exposed; BE) and B (Non-exposed; NE). These both groups were further subdivided into subgroups (A1 and A2, B1 and B2). Subgroups A1 and B1 received Ovsynch GnRH at day 0 followed by PGF2α day 7^th and second dose of GnRH at day 9^th. Whereas, in subgroups A2 and B2, estrus was synchronized with double PGF2α protocol at interval of 11days. The groups did not show differ (P>0.05) estrus response but found high in A1 (80%) than A2 (70%), B1 (70%) and B2 (60%). Estrus duration differs significantly (P<0.05) among groups. The progesterone level in group A1 and B1 increased from day 0 (1.25±0.095 and 1.08±0.11ng/ml, respectively) to 7^th (2.25±0.363 and 2.57±0.281ng/ml, respectively). Similarly in group A2 and B2 it was increase from day 0 (1.11±0.14 and 1.03±0.11ng/ml, respectively) to 11^th (1.25±0.17 and 1.39±0.14ng/ml, respectively). While at 18^th and 21^st day, the progesterone level (ng/ml) in A1 (1.38±0.152) and A2 (1.35±0.16) found high compared to B1 (1.12±0.143) and B2 (1.09±0.166) groups. Pregnancy rate was high in group A1 (60%) and A2 (50%) than B1 (50%) and B2 (40%), but the differences existed non-significant. Present study concludes that biostimulation with ovsynch protocol can effectively be used to induce cyclic activities and increases estrus response and fertility rate in Kundhi buffalo.

Key words: Artificial Insemination, Biostimulation, Estrus synchronization, Kundhi buffalo