July 2015 Vol. 3 No.6

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Zitouna N
Marghali S

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Original Research Article

Characterization of Sulla species via SRAP markers: polymorphism and systematic analyses

Zitouna Nadia, Gharbi Maroua, Chennaoui Houda, Touati Asma, Fadhlaoui Imen, Trifi-Farah Neila and Marghali Sonia*

Laboratory of Molecular Genetics, Immunology and Biotechnology, Faculty of Sciences of Tunis. University of Tunis El Manar - Campus
Universitaire, El Manar 2, 2092, Tunisia.

*Corresponding Author’s E-Mail: soniamarghali@yahoo.fr
Tel: +216 71 87 26 00
Fax: +216 70 86 04 32

Accepted July 15, 2015

Abstract

The use of molecular markers is effective to evaluate the genetic variation. The chosen biological material in this study is the Legume genus Sulla, which is threaten by an ecologic dequilibrium, the genetic erosion. Sequence-related amplified polymorphism (SRAP) markers were used to assess the genetic diversity and to schematize the relationships among and between the species of Sulla, endemic to mediterranean provinces. Twelve SRAP primer combinations generated 94 bands and showed a high inter-specific variability. Actually, (Φpt) value is relatively high 0.477 and the AMOVA analysis revealed that 62% of the diversity is inter-specific. These results were confirmed by the topology of the (NJ) cladogramm. The systematic examination of the genus of Sulla revealed the relatedness of the north african species, suggesting that the Maghreb is a hotspot of diversity. The use of these co-dominant markers allowed us to estimate the genetic diversity and then to improve the adaptability of the crops in the Mediterranean soils. SRAP markers could also be anchored to virtual Linkage Groups and then used for gene mapping.

Keywords: Sulla, Genetic diversity, SRAP, Phylogeny

Abbreviation

AFLP: Amplified Fragment of Length Polymorphism
AMOVA: Analysis of Molecular Variance
ISSR: Inter Sequence Simple Repeats
MCMC: Markov Chain Monte Carlo
ORFs: Open Reading Frames
PCoA: Principal Coordinate Analysis
QTL: Quantitative Trait Loci
RFLP: Restriction Fragment of Length Polymorphism
SRAP: Sequence Related Amplified Polymorphism
SSR: Simple Sequence Repeats