February 2017 Vol. 5 No.2

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Original Research Article

Practical Genetic Study Confirming and Identifying the Horizontal Gene Transfer (HGT) of Bacterial DNA (16S rDNA Gene) Integrated in the DNA of Cancer Patients

Munaff J. Abd Al-Abbas^* and Lamyaa A. Abdul-Ridha

Department of Biology, College of Science, University of Basrah, Basrah, Iraq

*Corresponding Author's Email: munaffjawdat@yahoo.com. Tel:+9647810069700

Accepted January 29, 2017

Abstract

Human DNA was extracted from 96 patients with hematological malignancies and from 26 healthy individuals, between 2014-2015. 72 (75%) bacterial 16S rDNA genes were detected in the DNA of patients with hematological malignancies, but none were detected in the 26 healthy individuals. Thirty-one Gram-negative bacteria were responsible for the majority of bacterial DNA integrations (43.05%), 5 Stenotrophomonas maltophilia and 5 Massilia timonae were identified as more abundant (6.94% for each) followed by 4 Methylobacterium lusitanum (5.55%) and 2 Methylobacterium rhodesianum (2.77%). Only 12 gram-positive bacteria were identified (16.66%), 2 Leuconostic lactis and 2 Bacillus endophyticus (2.77% for each) were the most common species. However, the expression test (cDNA) showed no result for the integrated 50 16S rDNA genes compared with 50 positive for IL-10-819 gene (100%). Interestingly, Random Amplified Polymorphic DNA for Bacillus cereus strains appeared only one case of cancer patient (1.38%) has the same strain integrated in the DNA and in his blood. This is the first practical study for detecting the bacterial DNA in the human DNA, Gram negative are the predominant bacteria including Stenotrophomonas maltophilia, Massilia timonae and Methylobacterium lusitanum. There was no expression of bacterial HGT in human.

Keywords: Bacteria, Cancer, HGT, Human, Integration